Scientific Publications

A list of scientific publications where I am one of the authors:



CO2-based Production of Phytase from Highly Stable Expression Plasmids in Cupriavidus necator H16

Published: January 2024

Cloning Cultivation

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Abstract
Existing plasmid systems offer a fundamental foundation for gene expression in Cupriavidus necator; however, their applicability is constrained by the limitations of conjugation. Low segregational stabilities and plasmid copy numbers, particularly in the absence of selection pressure, pose challenges. Phytases, recognized for their widespread application as supplements in animal feed to enhance phosphate availability, present an intriguing prospect for heterologous production in C. necator. The establishment of stable, high-copy number plasmids that can be electroporated would support the utilization of C. necator for the production of single-cell protein from CO2.