Scientific Publications
A list of scientific publications where I am one of the authors (also listed on Google Scholar):
Effects of feeding strategies on culture performance and product quality in NISTCHO
Systems Biology and Applications · March 2026
Abstract
Monoclonal antibody N-glycosylation is a critical quality attribute influencing
therapeutic safety and efficacy, and is strongly influenced by bioprocess
design. NISTCHO, a publicly available Chinese hamster ovary producer cell line,
is increasingly encouraged for use as a reference system. However, the impact
of feeding strategies on cellular performance and N-glycosylation has not been
assessed. Here, we applied multivariate analysis of compositional N-glycan data
to assess how feeding strategies influence N-glycan composition of cNISTmAb. We
varied feeding strategies in frequency, glucose supply, and galactose/manganese
supplementation. Feeding frequency had minimal impact on quality attributes but
strongly affected culture performance, with every-other-day feeding improving
titers and cell-specific productivity. High glucose availability supported
growth and productivity. Low glucose strategies reduced titers and shifted
N-glycosylation towards non-galactosylated and fucosylated species, despite
lactate accumulation remaining within favorable ranges. Galactose and manganese
consistently increased antibody galactosylation, with galactose additionally
serving as an auxiliary carbon source, extending cell viability. Importantly,
mAb glycation remained stable across all feeding strategies at harvest.
Overall, these results demonstrate that feed composition and timing can be used
to tune both cellular performance and mAb glycosylation, establishing NISTCHO
as a robust benchmark for standardized process-quality studies.
CO2-based Production of Phytase from Highly Stable Expression Plasmids in Cupriavidus necator H16
Microbial Cell Factories · January 2024
Abstract
Existing plasmid systems offer a fundamental foundation for gene expression
in Cupriavidus necator; however, their applicability is constrained by the
limitations of conjugation. Low segregational stabilities and plasmid copy
numbers, particularly in the absence of selection pressure, pose challenges.
Phytases, recognized for their widespread application as supplements in animal
feed to enhance phosphate availability, present an intriguing prospect for
heterologous production in C. necator. The establishment of stable, high-copy
number plasmids that can be electroporated would support the utilization of
C. necator for the production of single-cell protein from CO2.